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2EP Stocks

 
To facilitate high-resolution mapping, we constructed a set of lines with two EP elements each, inserted at a distance of 0.5 - 2.2 Mb (2EP stocks). We chose lines in which the associated white+ marker conferred only a pale-red eye color, so that chromosomes carrying two EP insertions can be detected by their dark red eyes. We recombined adjacent EP insertions to place them in cis, so that it is not necessary in mapping experiments to first recombine one of these markers onto the mutant chromosome. Our collection now comprises 62 2EP stocks, with sparse coverage of chromsome arm 3R but with excellent coverage of the four other major chromosome arms. All 2EP lines are available on request.
Number of 2EP stocks per chromosome arm
 
Chrom. arm# of 2EP stocks
X16
2L16
2R15
3L12
3R3
 
EP insertions
The table of 2EP stocks with insertions and their positional information is also available as Excel file (with Rel.5 or Rel.4 coordinates).
 
2EP stockChrom.EP element 1EP element 2Cytological region 1Cytological region 2Location 1 (Rel.5)Location 2 (Rel.5)Distance (Mb)
2EP_X01 X EP1421 EP1419 1A5-6 2A1-2 386595 1293933 0.91
2EP_X02 X EP1419 EP1605 2A1-2 3A1-2 1293933 2248376 0.95
2EP_X03 X EP1605 EP1316 3A1-2 4B1-2 2248376 4213539 1.95
2EP_X04 X EP1316 EP496 4B1-2 5A11-12 4213539 5574021 1.36
2EP_X05 X EP496 EP1377 5A11-12 6B2-3 5574021 6443852 0.87
2EP_X06 X EP1377 EP1340 6B2-3 7A1 6443852 7089130 0.65
2EP_X07 X EP1340 EP446 7A1 8A1-2 7089130 8575660 1.49
2EP_X08 X EP446 EP556 8A1-2 9B5-7 8575660 10184224 1.61
2EP_X09 X EP556 EP1537 9B5-7 10A4 10184224 10986547 0.8
2EP_X10 X EP1537 EP1451 10A4 11A1-2 10986547 11898005 0.9
2EP_X11 X EP1451 EP805 11A1-2 12A9-B2 11898005 13536085 1.64
2EP_X12 X EP805 EP1305 12A9-B2 13A5 13536085 14917914 1.38
2EP_X13 X EP1305 EP1522 13A5 14A9 14917914 15985725 1.07
2EP_X14 X EP1522 EP1625 14A9 15B1 15985725 16807594 0.82
2EP_X15 X EP1625 EP1357 15B1 16B10 16807594 17545549 0.74
2EP_X18 X EP1439 EP1525 18A4 19D1-2 19047666 20339345 1.22
2EP_2L01 2L EP456 EP2506 21A4 22B8 67010 1737426 1.67
2EP_2L02 2L EP2506 EP2099 22B8 23A3 1737426 2753202 1.02
2EP_2L03 2L EP2099 EP2350 23A3 23F1 2753202 3478411 0.73
2EP_2L04 2L EP2350 EP2323 23F1 25B1-2 3478411 4892125 1.41
2EP_2L05 2L EP2323 EP2273 25B1-2 26B5 4892125 6054256 1.16
2EP_2L06 2L EP2273 EP2454 26B5 27B1 6054256 6787433 0.73
2EP_2L07 2L EP2454 EP2419 27B1 28B2 6787433 7576408 0.79
2EP_2L09 2L EP2517 EP2569 29B2 29F6-7 8362514 8989213 0.63
2EP_2L10 2L EP2569 EP2465 29F6-7 30F6 8989213 10003132 1.01
2EP_2L11 2L EP2465 EP2478 30F6 32D2 10003132 11067009 1.06
2EP_2L12 2L EP2478 EP2377 32D2 33B13 11067009 12046132 0.98
2EP_2L13 2L EP2377 EP509 33B13 34A1 12046132 12822917 0.78
2EP_2L15 2L EP2039 EP1224 35A1 36A5 14409607 16588062 2.17
2EP_2L16 2L EP1224 EP2568 36A5 37B1 16588062 18823539 2.24
2EP_2L17 2L EP2568 EP623 37B1 37F1 18823539 19547011 0.72
2EP_2L18 2L EP623 EP2406 37F1 38D4 19547011 20829619 1.27
2EP_2R01 2R EP2189 EP2179 42B 43E4 2389404 3510556 1.12
2EP_2R02 2R EP2179 EP2233 43E4 44E3 3510556 4623287 1.11
2EP_2R03 2R EP2233 EP768 44E3 45B3 4623287 5073954 0.45
2EP_2R04 2R EP768 EP2603 45B3 46B4 5073954 5724105 0.65
2EP_2R05 2R EP2603 EP2079 46B4 47A13-B1 5724105 6505058 0.78
2EP_2R20 2R EP2079 EP622 47A13-B1 48A3 6505058 7503509 1
2EP_2R21 2R EP622 EP2386 48A3 50A14 7503509 9393966 1.89
2EP_2R09 2R EP2386 EP2041 50A14 50F1 9393966 10194722 0.8
2EP_2R10 2R EP2041 EP2411 50F1 51F4 10194722 11196859 1
2EP_2R11 2R EP2411 EP2578 51F4 52D15 11196859 11919425 0.72
2EP_2R14 2R EP2484 EP2498 53F8-9 54E1-2 12963815 13606937 0.64
2EP_2R16 2R EP2190 EP628 55E1-2 56F11 14499350 16139917 1.64
2EP_2R17 2R EP628 EP2395 56F11 57E1 16139917 17266972 1.12
2EP_2R18 2R EP2395 EP2184 57E1 59A1-2 17266972 18576579 1.31
2EP_2R19 2R EP2184 EP506 59A1-2 60B4-5 18576579 19916330 1.34
2EP_3L01 3L EP3643 EP387 61B2-3 62B12 247002 1586436 1.34
2EP_3L02 3L EP387 EP3041 62B12 63C1 1586436 3250614 1.66
2EP_3L03 3L EP3041 EP3676 63C1 64A5 3250614 4059828 0.81
2EP_3L04 3L EP3676 EP3639 64A5 65A10 4059828 6249842 2.19
2EP_3L05 3L EP3639 EP3389 65A10 66A17 6249842 7856182 1.61
2EP_3L06 3L EP3389 EP3336 66A17 67B1-2 7856182 9370469 1.51
2EP_3L07 3L EP3336 EP3688 67B1-2 68A4 9370469 11063637 1.69
2EP_3L09 3L EP3636 EP3557 69A5 70A2 12271126 13220867 0.95
2EP_3L10 3L EP3557 EP487 70A2 71A3 13220867 14962343 1.73
2EP_3L12 3L EP917 EP3444 72D9 73E4-5 16157382 17048916 0.89
2EP_3L15 3L EP3660 EP3273 75E4 77C3 18891418 20446381 1.52
2EP_3L16 3L EP3273 EP3230 77C3 79D2 20446381 22129145 1.68
2EP_3R10 3R EP3715 EP1100 89E8-11 91A2 12944862 14224947 1.28
2EP_3R11 3R EP1100 EP629 91A2 91F2 14224947 15006039 0.78
2EP_3R16 3R EP3540 EP3065 95B9-C4 96A6-7 19748774 20369653 0.62
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