The Flysnp Project
The FlySNP project aims to provide the information and technical resources to support high-throughput positional cloning in Drosophila melanogaster. These resources include a high-density genome-wide map of single nucleotide polymorphisms (SNPs), and inexpensive, high-throughput assays for SNP genotyping.
The specific aims were as follows:
1. To establish a map of >2200 SNP marker loci in the Drosophila genome.
These SNP markers have been identified in several commonly used genetic strains. The FlySNP project identified SNP markers within the sequenced, euchromatic regions of the X, 2nd and 3rd chromosomes. The average distance between SNPs is about 50 kb.
2. To establish robust, high-throughput assays for SNP genotyping.
Assays have been established using PCR, microarray and mass-spectrometry methods. The tag-array mini-sequencing (TAMS) approach has proven to be an especially fast and reliable method for SNP genotyping.
For latest results see also
"High-resolution, high-throughput SNP mapping in Drosophila melanogaster"
Doris Chen*, Annika Ahlford*, Frank Schnorrer*, Irene Kalchhauser, Michaela Fellner, Erika Virágh, István Kiss, Ann-Christine Syvänen & Barry J. Dickson
Nature Methods, 2008, Vol.5(4), pp323 - 329.
These SNP markers have been identified in several commonly used genetic strains. The FlySNP project identified SNP markers within the sequenced, euchromatic regions of the X, 2nd and 3rd chromosomes. The average distance between SNPs is about 50 kb.
Assays have been established using PCR, microarray and mass-spectrometry methods. The tag-array mini-sequencing (TAMS) approach has proven to be an especially fast and reliable method for SNP genotyping.
Doris Chen*, Annika Ahlford*, Frank Schnorrer*, Irene Kalchhauser, Michaela Fellner, Erika Virágh, István Kiss, Ann-Christine Syvänen & Barry J. Dickson
Nature Methods, 2008, Vol.5(4), pp323 - 329.
This project was funded by the European Union.